Fig. 4

Behavioral profiling of modifiers of acoustic startle habituation (a) Hierarchical clustering of behavioral profiles across the 26-endpoint assay (x-axis) is presented for twenty-four reference chemicals that impair or accelerate acoustic startle habituation (ASH1). Heatmap colors, based on effect sizes (SSMD, strictly standardized median difference), indicate deviations from the median control phenotype. Red and blue hues represent increased and reduced motor activity, respectively (n = 32–36 larvae). (b) MDS representation of pairwise distances between behavioral profiles based on data shown in a. Fill color indicates deviation from the median control phenotype in the ASH1 endpoint where red and blue reflect reduced and enhanced habituation, respectively. Median motor activity (y-axis) of vehicle control (0.4 % DMSO, grey) and (c) Ketamine (44.8 µM, red), (d) ML-109 (7.8 8 µM, red), (e) SB4 (44.8 µM, red) exposed zebrafish larvae over 30 sequential (ISI = 1 s) high-intensity acoustic stimuli (x-axis) (shaded envelope represents the 95 % confidence interval).