Figure 6

Histological analysis of hepatic tissue after 6 weeks of consumption of banaba (BNB, 0.1% w/w), policosanol (PCO, 0.1% w/w), and mixture of the banaba and policosanol (BNB+PCO, each 0.1%, w/w) supplemented with high cholesterol (HC, 4% w/w) and high galactose (HG, 30% w/w) diet. (A) Hematoxylin and eosin (H&E) staining, (B) oil red O (ORO) staining, (C) immunohistochemical analysis to detect interleukin 6 (IL-6), and (D) red conversion of the IL-6-stained area (to improve the visibility) using brown color threshold value 20–120 employing Image J software (https://imagej.net/ij, 1.53 version, accessed on 6 June 2023). (E) Quantification of H&E-stained area and neutrophil counts. (F,G) Quantification of ORO, and IL-6-stained area. The ** and *** underscore the significant variance at p < 0.01, p < 0.001 (for H&E, ORO, and IL-6-stained area), while ^### underscore the statistical difference at p < 0.001 (for neutrophil counts) vs. HC+HG group. The ^†, ^††, and ^††† highlight statistical differences at p < 0.05, p < 0.01, and p < 0.001 (for H&E and IL-6-stained area), while ^‡ represents the statistical difference at p < 0.05 (for neutrophil counts) vs. BNB+PCO group. The “ns ” highlights the non-significant difference vs. HC+HG group.