Figure 8

Comparison of brain lipid binding protein (BLBP), 4-hydroxynoneal (4-HNE) levels and cellular senescence in the zebrafish brain consuming banaba (BNB, 0.1% w/w), policosanol (PCO, 0.1% w/w), and mixture of the banaba and policosanol (BNB+PCO, each 0.1% w/w) amalgamated in high cholesterol (HC, 4% w/w) and high galactose (HG, 30% w/w) diet. (A,B) immunohistochemical (IHC) staining for the detection of brain lipid binding proteins (BLBPs) and lipid oxidize product 4-hydroxynoneal (4-HNE), (C) merged images obtained from BLBP and 4-HNE staining. (D,E) Senescent associated β-galactosidase (SA-β-Gal) staining at 40× and 100× magnification, respectively, the red arrow indicates senescent-positive cells, (F) red conversion of SA-β-Gal stained area (to improve the visibility) determined at blue color threshold value 0–120 employing Image J software. (G,H) Assessment of BLBP and 4-HNE fluorescent intensities, respectively. (I) Assessment of SA-β-Gal positive cells. The **, and *** underscore the significant variance at p < 0.01, and p < 0.001, vs. HC+HG group. The ^† and ^†† highlight significant variance at p < 0.05 and p < 0.01 vs. BNB+PCO. The “ns” highlights the non-significant difference.