Fig. 1

sptA inhibits growth and induces apoptosis in NSCLC cells. (a) The inhibitory effects of sptA on the growth of NSCLC cell lines and 16-HBE cells were evaluated with a CCK-8 assay (n = 5). (b) The inhibitory effects of sptA on the growth of NSCLC cell lines and 16-HBE cells were detected with an RTCA (n = 5). (c, d) Colony formation assays of NSCLC cell lines treated with sptA (n = 5). (e–g) Cell growth was assessed and imaged using high-content analysis (n = 5). The (f) area or (g) roundness of the cells was analysed using Harmony software (n = 5). Scale bar = 100 μm. (h, i) Cells were treated with sptA for 24 h and then asessed using annexin V/PI staining and flow cytometry (n = 5). (j) Cells were treated with sptA for 24 h, after which Western blot was performed (n = 5). (k, l) A549 xenograft zebrafish were treated with sptA or cisplatin at the indicated concentrations and observed at 2 dpi (n = 10). The intensity of red fluorescence is proportional to the tumour size. Data shown are individual values, with means ± SD. *P < 0.05, significantly different from control (0 nM sptA). DDP, cisplatin.