Figure 5

Microglial activation and leukocyte migration in the OB following 6-OHDA injection. (A–E) Lymphocyte cytosolic protein 1 (Lcp1) immunohistochemistry in sagittal sections of the OB from controls, 1 dpi, 3 dpi, 7 dpi, and 6-OHDA with the anti-inflammatory drug pranlukast (PRAN). Whole bulb (A) and magnified views of: (B) middle region of the OB, (C) dlG cluster region, (D) ventral region, and (E) the interphase between the telencephalon and the OB. Yellow: Lcp1; blue: DAPI. Scale bars: 100 µm in (A); 20 µm in (B–E). (F) Quantification of Lcp1+ cells in OB sections from (A) (n = 4–5). ANOVA: F (4, 17) = 10.66, p = 0.0002. ctrl vs. 1 dpi p = 0.0074, ctrl vs. 3 dpi p = 0.0151, ctrl vs. 7 dpi p = 0.0028, 1 dpi vs. 7 dpi p = 0.0028, 1 dpi vs. PRAN p = 0.0019, 3 dpi vs. 7 dpi p = 0.0061, 3 dpi vs. PRAN p = 0.0046. Box plots indicate mean (+), quartiles (boxes) and range (whiskers). One-way ANOVA, * p < 0.005, ** p < 0.001.