Figure 4

6-OHDA injections lead to astroglial activation in the OB. (A–C) Glial fibrillary acidic protein (GFAP) immunohistochemistry in sagittal sections of the OB from controls, 1 dpi, 3 dpi, 7 dpi, and 6-OHDA with the anti-inflammatory drug pranlukast (PRAN). Whole bulb (A) and magnified views of: (B) the olfactory nerve and anterior OB, and (C) the dorsolateral (dlG) glomerular cluster (indicated by dotted lines). GFAP+ staining along the olfactory nerve layer (ONL) is indicated with white arrowheads. Green: GFAP; blue: DAPI. Scale bars: 100 µm in (A); 50 µm in (B,C). (D) Quantification of GFAP O.D. in OB sections from (A) (n = 5–9). ANOVA: F (4, 27) = 8.478, p = 0.0001. ctrl vs. 1 dpi p = 0.0306, ctrl vs. 3 dpi p < 0.0001, ctrl vs. 7 dpi p = 0.0275. 1 dpi vs. 3 dpi p < 0.0001. 3 dpi vs. PRAN p = 0.0017. Box plots indicate mean (+), quartiles (boxes) and range (whiskers). One-way ANOVA, * p < 0.005, ** p < 0.001, **** p < 0.0001.