The s1171tEt line labels RSNs in the nMLF and is glutamatergic (vglut2) across development. A, Dorsal and sagittal MIPs from an example s1171tEt fish (of n = 12) at 6 dpf with RSNs labeled via retrograde dye injection. Scale bar 100 μm. B, Close ups at several planes to illustrate overlap between s1171tEt line and backfill, note the four main RSNs as indicated by red triangles. Scale bar 25 μm. C, Close ups at several planes of exemplary fish to show considerable overlap between s1171tEt and vglut2 (n = 8), particularly in RSNs. No overlap between s1171tEt and vglut1 (n = 7), chata (n = 8), gad1b/2 (n = 8), or glyt2 (n = 7). Scale bar 25 μm. D, vglut1 and vglut2 expression in exemplary 4-week-old juvenile fish (n = 4). Left) Two planes in dorsal view, scale bar 100 μm. Right) Matching transverse view, scale bar 50 μm. Blue triangles indicate blood vessels, red triangles point toward region of nMLF. For more examples of glutamatergic expression patterns in 4-week-old juvenile fish, see Extended data Figure 5-1.
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