Figure 2.

The nefma line labels all RSNs as well as cranial nerve nuclei. A, MIPs from dorsal and sagittal views of an exemplary nefma fish (n = 10) at 6 dpf with RSNs labeled via retrograde dye injection. B, Close ups at several planes to illustrate overlap between nefma line and backfill, indicated by red triangles. Putative CaD and CaV cells indicated by blue triangle. C, MIPs from dorsal and sagittal views of a nefma fish (n = 24) at 6 dpf with vglut2 mRNA expression. D, Close ups at several planes to illustrate considerable overlap between nefma line and vglut2. E, MIPs from dorsal and sagittal views of a nefma fish (n = 16) at 6 dpf with chata mRNA expression. F, Close ups at several planes to illustrate cranial nerves III–VII, IX–X, and OLe are labeled in the nefma line. For A, C, and E, scale bar 100 μm, for B, D, and F, scale bar is 25 μm. For GABAergic (gad1b/2), glycinergic (glyt1 and glyt2), or glutamatergic (vglut1) expression in neurons labeled by the nefma line, see Extended data Figure 2-1.