Figure 3.

The calca^ccu75Et line labels only IL-RSNs and closely matches the vsx2 line. A, MIPs from dorsal and sagittal views of an exemplary calca^ccu75Et fish (of n = 12) at 6 dpf with RSNs labeled via retrograde dye injection. B, Close ups at several planes to illustrate overlap between calca^ccu75Et line and backfill. Note that only IL-RSNs are present in calca^ccu75Et as indicated by red triangles. C, MIPs from dorsal and sagittal views of an exemplary calca^ccu75Et fish (of n = 8) at 6 dpf with vglut2 mRNA expression. D, Close ups at several planes to illustrate overlap between calca^ccu75Et line and vglut2, particularly of the RSNs as well as a rostro-caudal glutamatergic (vglut2) stripe in the dorsal hindbrain. E, Single planes from dorsal (105 μm) to ventral (180 μm) at 15 μm intervals in calca^ccu75Et, vsx2, and composite. For both top and middle panels, images are from two single fish (of n = 12 each) that were registered to a common reference brain (tERK). For A, C, and E, scale bar 100 μm, for B and D, scale bar 25 μm. For cholinergic (chata), GABAergic (gad1b/2), or glycinergic (glyt1 and glyt2) expression in neurons labeled by the calca^ccu75Et line, see Extended data Figure 3-1.