Figure 1

Effects of Pyripyropene O (PyrO) on the proliferation and migration of prostate cancer cells. (a) Chemical structural formula of Pyripyropene O (PyrO). (b) Effect of PyrO on cell viability. Cell viability was measured by the MTT assay after treatment of prostate cancer cells and normal prostate epithelial cells with different concentrations of PyrO (0–40 μM) for 72 h. (c,d) Effect of PyrO on clone formation in PC-3 cells. PC-3 cells were treated with PyrO (0–10 μM) for 14 days, then stained with crystal violet, and then photographed to observe the effect of PyrO on the ability of PC-3 cells to form clonal colonies. (e–g) Effect of PyrO on PC-3 three-dimensional proliferation. After treating PC-3 cell spheres inoculated in U-plates with PyrO (0–40 μM) for 72 h, live and dead cells were stained, and then the size of the cell spheres as well as the number of live and dead cells were observed by fluorescence microscopy. (h,i) Effect of PyrO on PC-3 cell migration. PC-3 cells were inoculated in CIM-plate 16 (5665817001, Agilent) plates, and the cells were treated with PyrO (0–10 μM) for 72 h. The migration of the cells was monitored by real-time cell analysis (RTCA). * p < 0.05, ** p < 0.01 vs. Ctrl.