Fig. 2
- ID
- ZDB-FIG-250528-20
- Publication
- Kaus-Drobek et al., 2025 - From discovery to potential application: engineering a novel M23 peptidase to combat Listeria monocytogenes
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Comparison of enzymatic lytic activity of StM23 and StM23_CWT enzymes on selected strains with different cell wall types as detailed in Table 1 in 50 mM Glycine pH 8.0 (upper panel) and in 50 mM Glycine pH 8.0 with 100 mM NaCl (lower panel). Lytic activity is expressed as reduction of initial OD595 (%) after 1 h of incubation with 1 µM enzyme. Values are adjusted by subtracting those from negative controls (bacterial suspensions incubated without added enzyme). Data are presented as mean ± SD of three replicates. Statistical analysis was performed using a two-way ANOVA with post hoc Dunnett’s multiple comparisons test performed in GraphPad Prism software (version 10.0.3). Significance levels are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. |