FIGURE

Fig. 2

ID

ZDB-FIG-250526-48

Publication

Kim et al., 2025 - Cohesin organizes 3D DNA contacts surrounding active enhancers in C. elegans

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Fig. 2

Acute depletion of cohesin and WAPL-1 from somatic cells. (A) C. elegans subunits of cohesin and its regulators. (B) Transgenic strains used in this paper. Exogenously inserted TIR1 is expressed in somatic cells using the eft-3 promoter. Endogenous smc-3 or wapl-1 are C-terminally tagged with degron-GFP. (C) DAPI and GFP images of L3 stage worms. Arrow indicates that the proteins in the germline are not degraded upon auxin treatment owing to TIR1 presence only in the somatic cells. Scale bar, 100 μm. (D) Western blot images of L3 stage worms with increasing auxin treatment duration. Anti-GFP antibody was used to show global depletion of the tagged protein. Antitubulin antibody was used as loading control. Asterisk indicates background bands. (E) Metaplot of ChIP-seq data grouped into different chromosomes. Anti-GFP antibody was used to show uniform depletion of SMC-3 and WAPL-1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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