FIGURE

Fig. 9

ID

ZDB-FIG-250521-20

Publication

Ford et al., 2025 - Nkx2.7 is a conserved regulator of craniofacial development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 9

Notch signaling is repressed by Nkx2.7 in patterning craniofacial cartilage and joints. ISH for jag1b at 26 hpf indicates enhanced expression (black arrow) in PA1 of nkx2.7−/− (n = 12) (C) compared to wild-type (n = 17) (A) and nkx2.5−/− (n = 15) (B) embryos. Dorsal views, anterior to the top. Scale bar, 50 μm. D Quantification of jag1b signal, detected by ISH, reveals statistically significant augmentation in PA1 in nkx2.7−/− compared to wild-type and nkx2.5−/− embryos (p < 0.0001). Mean and standard error of each data set are shown with analysis performed using an unpaired, two-tailed Student’s t-test. Alcian Blue staining in DMSO-exposed wild-type (n = 21) (E) and nkx2.7−/− (n = 26) (F) embryos and in DBZ-exposed nkx2.7−/− (n = 30) (G) embryos highlights lengthening and narrowing of anterior PA-derived cartilage elements in DBZ-exposed nkx2.7−/− embryos compared to DMSO-exposed wild-type and nkx2.7−/− embryos. Ventral views, anterior to the top. Scale, 100 μm. H Measurement of the M-Pq angle in embryos stained with Alcian Blue delineates a statistically significant decrease in the DBZ-exposed (n = 18) compared to the DMSO-exposed (n = 26) nkx2.7−/− embryos indicating a partial rescue towards the angle displayed in the DMSO-exposed wild-type embryos (n = 26). Mean and standard error of each data set are shown. Unpaired, two-tailed Student’s t-test yields p < 0.0001 between DMSO-exposed wild-type and nkx2.7−/− embryos, p = 0.0013 between DMSO-exposed wild-type and DBZ-exposed nkx2.7−/− embryos, and p < 0.0001 between DMSO-exposed and DBZ-exposed nkx2.7−/− embryos. I–L MF20 immunofluorescence depicts BMs in DMSO-exposed wild-type (n = 22) (I) and nkx2.7−/− (n = 18) (J) embryos and in DBZ-exposed nkx2.7−/− (n = 19) (K) embryos. No statistically significant difference in the number of PA-derived BMs is observed between DMSO- and DBZ-exposed nkx2.7−/− embryos employing an unpaired, two-tailed Student’s t-test. Ventral views, anterior to the top. Scale, 100 μm. M–O ISH for nkx3.2 at 52 hpf demonstrates reappearance of expression in PA1 of DBZ-exposed nkx2.7−/− (n = 9/9) (O) compared to DMSO-exposed nkx2.7−/− (n = 7/9) (N) embryos. Moreover, comparable expression domains are evident in DMSO-exposed wild-type embryos (n = 10/10) (M) and DBZ-exposed nkx2.7−/− embryos (O). Dorsal views, anterior to the top. Scale bar, 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.