Fig. 2

creb3l1TA+/TA+ fish exhibit no significant changes in size, skeletal morphology, or bone density. a Larvae from heterozygous creb3l1TA+/+ incrosses were genotyped at 7 dpf and the recovery ratios are presented. Nearly Mendelian ratios were obtained (χ2 analysis showed no statistical difference between predicted and observed ratios), with a slight decrease in the recovery of creb3l1TA+/TA+ animals. N = 202. b Body length of 6 mpf creb3l1+/+ and creb3l1TA+/TA+ fish was measured. An unpaired t-test with Welch’s correction indicates no significant difference in size. N = 21–34. Each data point represents an individual fish. c Representative μCT scans of creb3l1+/+ and creb3l1TA+/TA+ male fish at 3 and 8 mpf. No obvious defects in skeletal morphology were seen when visualized by 3D projections of the μCT scans. N = 9–11. d Bone density (mgHA/ccm) was measured by μCT scans in creb3l1+/+ and creb3l1TA+/TA+ fish at 3 and 8 mpf. Values for male fish are presented (analogous values were obtained for female fish, data not shown). An unpaired t-test with Welch’s correction shows no significant differences in bone density. N = 9–11. Each data point represents an individual fish.