FIGURE
Fig. 5
- ID
- ZDB-FIG-250424-7
- Publication
- Yamanaka et al., 2025 - Low-temperature embryo incubation suppresses off-target mutagenesis during CRISPR-Cas9 genome editing in medaka (Oryzias latipes) and zebrafish (Danio rerio)
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Fig. 5
The effect of early low-temperature treatment (LT) on germ line transmission. To examine the effect of LT on mutation introduction activity in germ cells, G0 founders were crossed with WT individuals to produce F1 founders. The presence of mutant alleles in F1 founders was assessed using Heteroduplex Mobility Assay (HMA). The ratio of F1 founders carrying heterozygous mutant alleles was calculated as the “Ratio of F1 founders with mutation allele’ (n = 5), based on 16–37 F1 eggs per G0 founder. Columns and error bars represent mean ± SE |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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