FIGURE

Fig. 4

ID

ZDB-FIG-250424-6

Publication

Yamanaka et al., 2025 - Low-temperature embryo incubation suppresses off-target mutagenesis during CRISPR-Cas9 genome editing in medaka (Oryzias latipes) and zebrafish (Danio rerio)

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Fig. 4

The effect of early low-temperature treatment (LT) on on-target and off-target mutation rates in zebrafish. The 28 °C group was cultured at 28 °C until hatching, while the LT group was cultured at 22 °C for 1 day, then shifted to 28 °C until hatching. No significant difference in mutation rate was observed between the 28 °C and LT groups for ywhaqa (n = 7–10). However, for Off-Y1, LT significantly reduced the mutation rate compared to 28 °C (n = 7–10). For Off-Y2, although the difference was not statistically significant, the mutation rate tended to be lower in the LT group(n = 7–10). N.S.: no significant difference, *: p < 0.05. Columns and error bars represent mean ± SE

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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