FIGURE

Fig. 2

ID

ZDB-FIG-250424-4

Publication

Yamanaka et al., 2025 - Low-temperature embryo incubation suppresses off-target mutagenesis during CRISPR-Cas9 genome editing in medaka (Oryzias latipes) and zebrafish (Danio rerio)

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Fig. 2

The effect of low-temperature incubation on on-target and off-target mutation rates. After the introduction of RGEN, embryos were cultured at 28 °C or 16 °C until Stage 25. Amplicon sequencing was performed for each region, and the mutation rate was calculated. A DJ-1 and Off-D (n = 10) B p4hb and Off-P (n = 10) C avt and Off-A (n = 8). While no significant difference in on-target mutation rates was observed between temperatures, off-target mutation rates were significantly reduced at 16 °C compared to 28 °C. N.S.: no significant difference, **: p < 0.01, ***: p < 0.001 Columns and error bars represent mean ± SE

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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