PUBLICATION
Low-temperature embryo incubation suppresses off-target mutagenesis during CRISPR-Cas9 genome editing in medaka (Oryzias latipes) and zebrafish (Danio rerio)
- Authors
- Yamanaka, T., Sogo, A., Maegawa, S., Kinoshita, M.
- ID
- ZDB-PUB-250326-6
- Date
- 2025
- Source
- Transgenic Research 34: 1515 (Journal)
- Registered Authors
- Maegawa, Shingo
- Keywords
- CRISPR-Cas9, Medaka, Off-target mutation, Temperature, Zebrafish
- MeSH Terms
-
- Mutation
- Embryo, Nonmammalian
- CRISPR-Cas Systems*
- Animals
- Gene Editing*/methods
- Cold Temperature
- Oryzias*/embryology
- Oryzias*/genetics
- Zebrafish*/embryology
- Zebrafish*/genetics
- Temperature
- Mutagenesis*
- PubMed
- 40131558 Full text @ Transgenic. Res.
Citation
Yamanaka, T., Sogo, A., Maegawa, S., Kinoshita, M. (2025) Low-temperature embryo incubation suppresses off-target mutagenesis during CRISPR-Cas9 genome editing in medaka (Oryzias latipes) and zebrafish (Danio rerio). Transgenic Research. 34:1515.
Abstract
Gene knockout using CRISPR-Cas9 is often employed in research aimed at elucidating gene functions in fish. However, CRISPR-Cas9 sometimes introduces unintended alterations, known as off-target mutations. These mutations can reduce the robustness of data during phenotypic analysis. In this study, we focused on the culture temperature, which is known to significantly influence mutagenesis, and examined whether low-temperature culture after introducing CRISPR-Cas9 into early embryos of medaka and zebrafish suppresses off-target mutations. Continuous incubation of medaka at 16 °C significantly reduced off-target mutation rates compared to those at 28 °C; the drawback is that it decreased the survival rate of medaka embryos. Therefore, low-temperature incubation was limited to early development in both zebrafish and medaka, and then the temperature was increased to 28 °C. Under these conditions, the mutation rates of the three off-target regions in medaka (Off-D, Off-P, and Off-A) significantly decreased, whereas those of the three target regions (DJ-1, p4hb, and avt) were unaffected. Similarly, the mutation rate of the zebrafish target region (ywhaqa) remained high, whereas the off-target (Off-Y1) mutation rate significantly reduced. Furthermore, this method effectively suppressed the germ line transmission of off-target mutations in medaka. This approach is effective to obtain more reliable data from the G0 generation of medaka and zebrafish and may reduce the screening effort required to remove individuals with off-target mutations in the F1 generation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping