FIGURE

Fig. 1 - Supplemental 1

ID

ZDB-FIG-250424-24

Publication

Scerbo et al., 2025 - In vivo targeted and deterministic single-cell malignant transformation

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Fig. 1 - Supplemental 1

Characterization of the double transgenic line Tg(?-actin:loxP-EOS-stop-loxP-KRASG12V-T2A-H2B-mTFP; ubi:Cre-ERT; myl7:EGFP). (A) A transgenic fish at 6 day post-fertilization (dpf) displays green EOS fluorescence throughout the body and a strong green fluorescence in the heart due to the additive expression of EGFP under a heart-specific (myl7) promotor, red arrowhead. (B) Upon global illumination (at 1 dpf) with an ~365 nm UV lamp in presence of caged cyclofen (cCYC), cyclofen is uncaged which releases CRE-ERT from its complex with cytoplasmic chaperones and results in floxing of EOS and ubiquitous expression of KRASG12V and H2B-mTFP. The embryos thus display blue fluorescence in the nuclei shown here at 1 hr post-induction (hpi). (C) Stable and ubiquitous expression of the blue H2B-mTFP fluorescent protein in zebrafish late embryos (3 dpf) and larvae (6 dpf). Note that in all pictures the asterisk (*) indicates the eye. Scale bars and body axes (a: anterior; p: posterior; d: dorsal; v: ventral) are shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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