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pycard is expressed in different tissues and blood cell types. (A) Each datapoint represents the expression level of pycard in a single organ collected from a single AB fish (no treatment) [n=3 (gut, kidney, testis, gonad), n=4 (spleen, gills, tailfin, eyes, skin, muscle, brain, swim bladder, heart, liver)]. (B) AB zebrafish infected with M. marinum (Mmar) (mean, 28 CFU; range, 21-34 CFU) display a decrease in pycard expression in their monocyte and granulocyte population. Fish were either infected with Mmar or mock injected with PBS. At 4 wpi, the fish were euthanized and their kidneys were collected for fluorescence-activated cell sorting. RNA was extracted from the sorted populations, and pycard expression was measured with qPCR and normalized to eef1a1/1 expression. Each sample contained kidneys from three fish, pooled (n=3-4). Both male and female fish were used in both experiments. The line indicates the median. *P(monocytes and granulocytes)=0.0286, Mann–Whitney, two tailed.
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