Fig. 5

Aggravated retinal vasculature alterations in DKO larvae and DKO adult zebrafish. (A) Representative confocal images of the hyaloid vasculature showed amplified alterations in DKO zebrafish larvae at 120hpf compared to WT, GLO1KO and ALDH3A1KO larvae. Red arrows indicated branch points, and yellow arrows indicated sprouts. White scale bar = 20 μm. (B) Quantification of increased branch points formation in the hyaloid vasculature in DKO larvae compared to WT, GLO1KO and ALDH3A1KO larvae, n = 41–67. (C) Quantification of increased vessel diameter in the hyaloid vasculature in DKO larvae compared to WT larvae, n = 41–67. (D) Quantification of sprouts formation in the hyaloid vasculature in DKO larvae, n = 41–67. (E) Representative confocal images of retinal vasculature showed increased sprouts in DKO adult zebrafish at 12 mpf. Red arrows indicated branch points, and yellow arrows indicated sprouts. White scale bar = 350 μm. (F) Quantification of increased sprouts formation in the retinal vasculature in DKO adult zebrafish, n = 18–23. (G) Quantification of branch points formation in the retinal vasculature in DKO adult zebrafish, n = 18–23. The bars indicate mean ± SD values. Statistical analysis was performed by one-way ANOVA or Kruskal-Wallis test. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. hpf, hours of post fertilization; mpf, months of post fertilization.