Fig 3

(A) Schematic diagram of the protocol to evaluate Enterobacter stability after feeding and re-inoculation. As described in the main text, initially germ-free fish were inoculated with GFP-labeled EN and fed UV-treated or control rotifers or left unfed for 3 days, starting at 4 or 6 dpf. Groups 2–4 were re-inoculated with dTomato-labeled EN on different days. (B–F) Final EN abundance in unfed larvae (cyan), larvae fed with UV-treated rotifers (pink), and larvae fed with untreated rotifers (gray). Circles represent data from larvae fed from 5 to 7 dpf and crosses from 7 to 9 dpf. (B) Fluorescent EN-GFP abundance in group 1 larvae assessed 1 day post-inoculation (before the start of feeding) and 4 days post-inoculation (after 3 days of feeding). (C–E) From groups 2, 3, and 4, total fluorescent EN abundance (GFP and dTomato) (C), EN-GFP abundance (D), and EN-dTomato abundance (E) 1, 2, or 3 days post EN dTomato inoculation. (F) Fraction of EN-GFP relative to the total EN abundance 1, 2, or 3 days post EN-dTomato inoculation.