Fig 4

(A) Schematic diagram of the protocol to evaluate the stability of multiple bacterial species in larvae fed until 13 dpf. Initially germ-free larvae were inoculated with GFP-labeled PS, AE, and EN at 4 dpf and split into two groups. Zebrafish in group 2 was re-inoculated with dTomato-labeled PS, AE, and EN at 8 dpf. Both groups were fed from 5 to 11 or 12 dpf and homogenized and plated at 12 or 13 dpf. (B) Fluorescent bacterial abundance per larva after 7 or 8 days of feeding. Pink corresponds to larvae fed with UV treated rotifers and gray to larvae fed with untreated rotifers. (C and D) Bacterial abundance of each inoculated per strain for fish in groups 1 (C) and 2 (D). (E) Total abundance of GFP-labeled bacteria, descended from initial colonizers, of each strain in larvae from group 2. (F) Total abundance of dTomato-labeled bacteria, descended from later colonizers, in larvae from group 2.