FIGURE

Fig. 3

ID

ZDB-FIG-250311-50

Publication

Peralta et al., 2024 - Endothelial calcium firing mediates the extravasation of metastatic tumor cells

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Fig. 3

Impairing Ca2+ signaling prevents metastatic extravasation (A) Schematic representation of the experimental design. Graph shows EC actin clustering for control (CTC ROIs from non-treated embryos) and nifedipine-treated embryos throughout the time lapse in arbitrary units (A.U., Mann Whitney test, p-value 0.0001; control: 3 embryos, 19 CTC- ROIs measured; nifedipine: 2 embryos, 18 CTC- ROIs measured for 6 hpi time-lapse). Images show representative examples of the analysis pipeline selections for CTC- and no-CTC- ROIs (yellow line) at 6 hpi for each condition. (B) Schematic representation of the experimental design. Graph shows the percentage of CTC extravasation in control and nifedipine-treated embryos at 21 hpi (Mann Whitney test, p-value 0.0001; control: 9 embryos; nifedipine-treated: 15 embryos). Data are represented as mean ± SD. Confocal z stack projections displaying representative examples of control and nifedipine-treated embryos. EC channel is displayed using inverted LUT to facilitate visualization. Zoom boxes show a single confocal plane to improve the visualization of intravascular (yellow asterisks) and extravascular (red arrowhead) CTCs.

Expression Data

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Antibody Labeling

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Acknowledgments

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