Dmrt1 regulates somatic growth in male zebrafish by mediating stat5b expression. (A,B) Gh mRNA levels in pituitary (A) and plasma GH levels (B) of WT and dmrt1−/− male zebrafish at 5 mpf. (C,D) Relative expression of stat5b mRNA (C) and Stat5b protein (D) in liver. Representative images of Western blot (top) and the quantitation (bottom; n = 3 per assay). (E) Binding site of transcription factor Dmrt1 in the stat5b promoter region predicted by JASPAR. (F) Schematic representation of the normal and mutational stat5b promoter luciferase constructs and their activities in dual-luciferase assay. Dmrt1 overexpression inhibited the luciferase activity of stat5b promoter in HEK-293T cells. The empty vector pcDNA3.1+ and pGL3-basic were transfected as the control. (G,H) The body length and body weight of male self-bred offspring of dmrt1 and stat5b double heterozygotes at 3 mpf. The groups with different letters indicate that they are statistically significant (p < 0.05). (I) The body length and body weight of male dmrt1 transgenic line at 3 mpf (n = 20). (J) Relative expression of stat5b mRNA in liver of dmrt1 transgenic male fish. Data are represented as mean ± SD (p < 0.05 *, p < 0.01 **; p < 0.001 ***; ns, no significant).
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