Fig. 5

Reduced body axis elongation in vangl2 mutant is associated with altered dorsal cell movement.

A Transmitted light lateral view of 10SS vangl2 mutant and sibling embryos—the vangl2 mutant has a shorter anterior-posterior axis than sibling (9/9 vangl2^−/− and 13/13 siblings analyzed). B Orthogonal 10 µm confocal projections of vangl2 mutant tailbud injected with H2B-RFP mRNA to label nuclei (white) showing the wide and flat shape. Representative image from 5 vangl2^−/− embryos analyzed. C Posterior body elongation speed is strongly reduced in vangl2 mutant embryos compared to sibling (N = 13 for siblings and N = 9 for vangl2^−/−; center line: mean; box: SE; whiskers: SD). D Average normalized velocity is reduced in vangl2 mutant dorsal tissue compared to wild type (For wild-type, n_t = 5214 from N = 10, and for vangl2, n_t = 5286 from N = 10; shading SE) E Maximum intensity projection of a dorsal view of a vangl2 mutant tailbud with tracked nuclei showing that there is no relative posterior movement of cells in dorsal tissue with respect to cells in ventral tissue. Representative image from 3 vangl2^−/− embryos analyzed. F 2D velocity field of vangl2 mutant ventral tissues show that the pattern of ventral cell movement is unaffected in the vangl2 mutant, whereas 2D velocity field of dorsal tissue shows that cells are converging on the midline with less posterior progression. G Fluidity Index pattern is unaffected in vangl2 mutants (average fluidity index N = 5 embryos) compared to wild type. Scale bar: 400 µm (A); 100 µm (B, E). Source data are provided as a Source Data file.