PUBLICATION
The physical roles of different posterior tissues in zebrafish axis elongation
- Authors
- Stooke-Vaughan, G.A., Kim, S., Yen, S.T., Son, K., Banavar, S.P., Giammona, J., Kimelman, D., Campās, O.
- ID
- ZDB-PUB-250222-11
- Date
- 2025
- Source
- Nature communications 16: 18391839 (Journal)
- Registered Authors
- Banavar, Samhita, Giammona, James, Kimelman, David, Kim, Sangwoo, Stooke-Vaughan, Georgina, Yen, Shou-Ting
- Keywords
- none
- MeSH Terms
-
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Body Patterning*/genetics
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- Mesoderm*/embryology
- Mesoderm*/metabolism
- Notochord*/embryology
- Notochord*/metabolism
- Animals
- Mutation
- Zebrafish*/embryology
- Zebrafish*/genetics
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism
- Gene Expression Regulation, Developmental
- Embryo, Nonmammalian/metabolism
- Morphogenesis/genetics
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 39984461 Full text @ Nat. Commun.
Citation
Stooke-Vaughan, G.A., Kim, S., Yen, S.T., Son, K., Banavar, S.P., Giammona, J., Kimelman, D., Campās, O. (2025) The physical roles of different posterior tissues in zebrafish axis elongation. Nature communications. 16:18391839.
Abstract
Shaping embryonic tissues requires spatiotemporal changes in genetic and signaling activity as well as in tissue mechanics. Studies linking specific molecular perturbations to changes in the tissue physical state remain sparse. Here we study how specific genetic perturbations affecting different posterior tissues during zebrafish body axis elongation change their physical state, the resulting large-scale tissue flows, and posterior elongation. Using a custom analysis software to reveal spatiotemporal variations in tissue fluidity, we show that dorsal tissues are most fluid at the posterior end, rigidify anterior of this region, and become more fluid again yet further anteriorly. In the absence of notochord (noto mutants) or when the presomitic mesoderm is substantially reduced (tbx16 mutants), dorsal tissues elongate normally. Perturbations of posterior-directed morphogenetic flows in dorsal tissues (vangl2 mutants) strongly affect the speed of elongation, highlighting the essential role of dorsal cell flows in delivering the necessary material to elongate the axis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping