FIGURE

Fig. 4

ID

ZDB-FIG-250225-52

Publication

Stooke-Vaughan et al., 2025 - The physical roles of different posterior tissues in zebrafish axis elongation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Body axis elongation proceeds in the absence of a notochord.

A Transmitted light lateral view of 10 SS noto sibling and mutant embryo – there is no obvious difference in overall embryo shape at this stage. B Posterior body elongation speed is no different in noto mutants compared to siblings (N = 4 for sib and N = 5 for noto^−/−; center line: mean; box: SE; whiskers: SD). C Orthogonal 10 µm confocal projections of noto mutant tailbud injected with H2B-RFP mRNA to label nuclei (white). There is no notochord, and the space usually filled by it is instead occupied by dorsal tissue and PSM. Representative image from 5 noto^−/− embryos analyzed. D Maximum intensity projection of dorsal view of noto mutant tailbud with tracked nuclei showing that tissue shear in the PSM and relative posterior movement of overlying dorsal tissue are comparable to wild type. Representative image from 3 noto^−/− embryos analyzed. E 2D velocity field of noto mutant is similar to wild-type apart from the absence of counter-rotating vortices at the MPZ—pPSM boundary. F Fluidity index shows no change from the wild-type pattern in noto^−/− (average fluidity index N = 5 embryos). Scale bar: 400 µm (A); 100 µm (C, D). Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.