Figure 9

Induction of apoptosis in PBMCs via MV internalization

(A and B) Flow cytometric analysis of Caspase 3/7 in PBMCs and Jurkat ± Anti-CD44 and ± EIPA exposed to AEC ± LPS_MV for 48h. Data are presented as MFI ratio over negative CTR.

(C–E and D–F) JC1 flow cytometric analysis to evaluate the ΔΨM of PBMCs and Jurkat ± EIPA exposed to AEC ± LPS_MV for 12-72h. Data were presented as MFI ratio over negative CTR. Values were normalized on the basal ΔΨM of activated PBMCs. Data (mean ± SD) represent 3 independent sets of experiments (n = at least 3 biological replicates in each group per set; each biological replicate assayed in at least 3 technical replicates). (A) ALL, ∗, ∗∗, ∗∗∗, and ∗∗∗∗ Statistically significant values between the different studied groups (p < 0.0001, p < 0.05, p < 0.01, p < 0.001, and p < 0.0001, respectively). (B) ∗, ^Δ, and ^▪p < 0.05 between the groups studied (PHA, DMSO, AEC ± LPS_MV, respectively). No apoptotic effect was recorded following AEC_MV mitochondria transfer into AEC (see Figure S1). One-way ANOVA was employed for comparing normally distributed data.