PUBLICATION
Amniotic epithelial Cell microvesicles uptake inhibits PBMCs and Jurkat cells activation by inducing mitochondria-dependent apoptosis
- Authors
- Cerveró-Varona, A., Prencipe, G., Peserico, A., Canciello, A., House, A.H., Santos, H.A., Perugini, M., Sulcanese, L., Takano, C., Miki, T., Iannetta, A., Russo, V., Mattioli, M., Barboni, B.
- ID
- ZDB-PUB-250219-6
- Date
- 2025
- Source
- iScience 28: 111830111830 (Journal)
- Registered Authors
- Keywords
- Cell biology, Functional aspects of cell biology, Immunology
- MeSH Terms
- none
- PubMed
- 39967871 Full text @ iScience
Citation
Cerveró-Varona, A., Prencipe, G., Peserico, A., Canciello, A., House, A.H., Santos, H.A., Perugini, M., Sulcanese, L., Takano, C., Miki, T., Iannetta, A., Russo, V., Mattioli, M., Barboni, B. (2025) Amniotic epithelial Cell microvesicles uptake inhibits PBMCs and Jurkat cells activation by inducing mitochondria-dependent apoptosis. iScience. 28:111830111830.
Abstract
Amniotic epithelial cells (AECs) exhibit significant immunomodulatory and pro-regenerative properties, largely due to their intrinsic paracrine functions that are currently harnessed through the collection of their secretomes. While there is increasing evidence of the role of bioactive components freely secreted or carried by exosomes, the bioactive cargo of AEC microvesicles (MVs) and their crosstalk with the immune cells remains to be fully explored. We showed that under intrinsic conditions or in response to LPS, AEC-derived MV carries components such as lipid-mediated signaling molecules, ER, and mitochondria. They foster the intra/interspecific mitochondrial transfer into immune cells (PBMCs and Jurkat cells) in vitro and in vivo on the zebrafish larvae model of injury. The internalization of MV cargoes through macropinocytosis induces hyperpolarization of PBMC mitochondrial membranes and triggers MV-mediated apoptosis. This powerful immune suppressive mechanism triggered by AEC-MV cargo delivery paves the way for controlled and targeted cell-free therapeutic approaches.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping