Fig. 6

Expression of chrna3tdTomato, chrnb4eGFP, and chrna5tdTomato in motor neurons. (A and B) Dorsal view of the hindbrain showed the co-expression of chrna3tdTomato/mnx1eGFP and chrna3tdTomato/chrnb4eGFP; dashed box region shown magnified for individual transgenic lines; circle shows the location of branchiomotor neurons labeled by mnx1eGFP. (C–E) Lateral view of the expression of chrna3tdTomato, chrnb4eGFP, and chrna5tdTomato positive cells overlapping with mnx1 expressing cells in motor neurons; dashed box region shown magnified for individual transgenic lines; arrow refers to the overlapped cell; asterisk refers to the motor neuron of mnx1GFP positive without the expression of chrn-gene. (F) Quantification of positive cells per segment in the spinal cord in chrna3tdTomato, chrnb4eGFP, and chrna5tdTomato crossed with mnx1eGFP/RFP, n = 5 fish for each transgenic line. (G) Quantification of primary motor neurons (pMN) per hemisegment in the spinal cord in chrna3tdTomato, chrnb4eGFP, and chrna5tdTomato, n = 5 fish for each transgenic line. (H) Transverse view of the expression of chrna5tdTomato labeling neurons in the spinal cord and their projections innervated muscles. (H1) Sketch of the projection pattern of the cell that is highly expressed with chrna5tdTomato and identified as the sl-type secondary motor neuron. (H2) Lateral view of a cell that highly expressed chrna5tdTomato. (I) Lateral view of the co-expression of chrnb4eGFP and chrna5tdTomato in the sl-type secondary motor neuron showed before. Scale bars equal 25 µm in (A) and (B), 10 µm in (C)–(E), 25 µm in (H2) and (I). Quantification data show mean values + standard deviation. C, caudal; D, dorsal; R, rostral.