Figure 5

Effect of oc genes depletion on zebrafish retinal organization. (A–E) Sagittal sections of zebrafish retinas at 72 hpf from the SoFa1 transgenic line. (F–J) Immunostaining for Zn-8 in RGCs, and (K–O) for SV2 in IPL and PRC axon terminus (white arrows) of zebrafish eye at 72 hpf. (A,F,K) STD MO, (B,G,L) oc1, (C,H,M) oc2, (D,I,N) oc-like and (E,J,O) triple oc-MO eye sections. Retinal populations are unaltered following STD MO injection (A,F,K). A reduction of RGC (G) and AC (B) is reported in oc1-MO retina, and milder alterations are visible in oc-like-MO (D,I,N) and oc2-MO (C,H,M) respectively. Triple oc-MO retina (E,J,O) shows severe alterations and reductions in the RGC and a complete absence of PRC axon terminals (white arrow). (P) Schematic representation of the retinal alterations. The reduced number of cells represented in this schematic drawing is just an illustration and is not quantitative. AC, amacrine cells; BP, bipolar cells, HC, horizontal cells; PRC, photoreceptor cells; RGC, retinal ganglion cells; GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer.