PUBLICATION

The Zebrafish Retina and the Evolution of the Onecut-Mediated Pathway in Cell Type Differentiation

Authors
Vassalli, Q.A., Fasano, G., Nittoli, V., Gagliardi, E., Sepe, R.M., Donizetti, A., Aniello, F., Sordino, P., Kelsh, R., Locascio, A.
ID
ZDB-PUB-250109-184
Date
2024
Source
Cells   13(24): (Journal)
Registered Authors
Aniello, Francesco, Donizetti, Aldo, Kelsh, Robert, Sordino, Paolo
Keywords
Danio rerio, Hnf6, comparative evolution, eye, genetic pathway, photoreceptor
MeSH Terms
  • Animals
  • Cell Differentiation*/genetics
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
  • Retina*/cytology
  • Retina*/metabolism
  • Zebrafish*/genetics
  • Gene Expression Regulation, Developmental
PubMed
39768162 Full text @ Cells
Abstract
Onecut/Hnf6 (Oc) genes play an important role in the proper formation of retinal cells in vertebrates, in particular horizontal, retinal ganglion and amacrine cells. However, it is not fully known how the unique and combined action of multiple Oc gene copies leads to the induction and differentiation of specific retinal cell types. To gain new insights on how Oc genes influence retina formation, we have examined the developmental role of oc1, oc2 and oc-like genes during eye formation in the non-mammalian vertebrate zebrafish Danio rerio. By using single and multiple morpholino knockdown of three zebrafish Oc genes we provide evidence for the independent and redundant role of each gene in the formation of photoreceptors and other retinal tissues. Through comparison of Oc genetic pathways in photoreceptor differentiation among chordates we demonstrate their mechanism of action through a series of conserved target genes involved in neural transmission.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping