FIGURE

Fig. 1

ID

ZDB-FIG-250109-102

Publication

Ribeiro et al., 2024 - Phenotypic screening in zebrafish larvae identifies promising cyanobacterial strains and pheophorbide a as insulin mimetics

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Screening for insulin mimetic activity in zebrafish larvae exposed to cyanobacterial fractions using the 2-NBDG assay. Percentage of glucose uptake relative to solvent control (DMSO 0.1%) was evaluated. 182 fractions were screened and fluorescence was quantified in the yolk sac (A) and in the eye (B). Fractions with an increase > 20% are shown in blue, and > 50% in green. Promising fractions from the initial screening were tested in an additional assay. Fractions marked in green increased significantly 2-NBDG uptake in the yolk sac (C) or eye (D). The data is represented as box-whisker plots from the fifth to 95th percentiles. Asterisks highlight significantly altered fluorescence intensities that indicate changes in 2-NBDG uptake (* p < 0.05), Kruskal-Wallis and Dunn’s test. E) Representative images from fluorescence microscopy: solvent control (DMSO, 0.1%); positive control (emodin, 10 µM) and fraction 57, 10 µg/mL.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.