ZFIN Image: Ribeiro et al., 2024, Fig. 1

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Fig. 1

Screening for insulin mimetic activity in zebrafish larvae exposed to cyanobacterial fractions using the 2-NBDG assay. Percentage of glucose uptake relative to solvent control (DMSO 0.1%) was evaluated. 182 fractions were screened and fluorescence was quantified in the yolk sac (A) and in the eye (B). Fractions with an increase > 20% are shown in blue, and > 50% in green. Promising fractions from the initial screening were tested in an additional assay. Fractions marked in green increased significantly 2-NBDG uptake in the yolk sac (C) or eye (D). The data is represented as box-whisker plots from the fifth to 95th percentiles. Asterisks highlight significantly altered fluorescence intensities that indicate changes in 2-NBDG uptake (* p < 0.05), Kruskal-Wallis and Dunn’s test. E) Representative images from fluorescence microscopy: solvent control (DMSO, 0.1%); positive control (emodin, 10 µM) and fraction 57, 10 µg/mL.

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