FIGURE

Fig. 3

ID

ZDB-FIG-241219-63

Publication

Li et al., 2024 - A potent and selective anti-glutathione peroxidase 4 nanobody as a ferroptosis inducer

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

(A) Molecular modeling of GPX4 and NBs 12E (blue), 4C (purple) and 5C (yellow). (B) RMSF of GPX4 within GPX4-12E or GPX4-5C complexes. (C) Molecular surface and zoomed-in views of interactions of the GPX4-12E complex. (D) Kinetic binding curves of GPX4 mutants (mutants 1, 2 and 3) and 12E determined by SPR. The arrow indicates that the concentrations of NBs increased from 1.95 nM to 500 nM. AllCys(-)GPX4U46C,S44A, Q45A, K48A, E50A, AllCys(-)GPX4U46C, I129A, L130A, K135A, W136A and AllCys(-)GPX4U46C, P155A, M156A, E157A were named GPX4 mutants 1, 2, and 3, respectively. (E) TR-FRET measurement for the affinity tests of GPX4 mutants and 12E. Left: schematic representation of the TR-FRET measurement. Middle: affinity binding curves of GPX4 mutants and 12E via TR-FRET. The results were repeated two times and presented as mean ± SD (n = 2). Right: the Kd values of GPX4 mutants and 12E via TR-FRET. Kd was obtained by fitting the relative FRET ratio with the ‘One site-total binding’ model in Prism 8.0. ****p < 0.0001 vs. the vehicle control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Chem Sci