FIGURE

Fig. 2.

ID

ZDB-FIG-241210-20

Publication

Khan et al., 2024 - Optimised methods to image hepatic lipid droplets in zebrafish larvae

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Fig. 2.

NR staining of LDs in live and fixed larvae shows increased steatosis incidence and severity in response to TM, ethanol and fasting. (A) Representative confocal images of hepatocytes (EGFP membrane marker; green) and LDs (magenta) in 120 hpf zebrafish untreated or treated with DMSO (vehicle), 350 mM ethanol (EtOH), 0.5 μg/ml TM from 96 to 120 hpf, and fasted larvae (7 dpf) stained with NR. The transgenic line Tg(fabp10a:CAAX-EGFP) was used to mark hepatocyte membrane. Scale bars: 20 μm. White arrowheads (LD) point to examples of LDs and yellow arrows (BV) point to blood vessels. Images are representative of 30 larvae from three clutches. (B) Steatosis incidence measured by percentage of samples having two or more LDs in a single z-plane through the middle of the liver (ns, not significant; *P<0.05, ***P<0.001, ****P<0.0001 by unpaired two-tailed Student's t-test, n=3 clutches, 30 larvae). (C) Steatosis severity quantified by the number of LDs divided by the total area of the liver in a single z-plane for each treatment condition (ns, non-significant; *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 by unpaired two-tailed Student's t-test, n=3 clutches, 30 larvae).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.