Figure 6

Distribution of tle orthologs in nontuberculous mycobacteria and correlation with the presence of 6-d-Tal.A, phylogenetic tree reconstructed using already available tle sequences. Nonmycobacterial species and mycobacterial species are represented in pink and blue areas, respectively. Green, yellow, and red dots label slowly-growing, intermediately-growing, and rapid-growing mycobacteria, respectively. B, TLC analysis of the polar lipid fraction of a selected panel of NTM clinical strains, representative from two independent experiments. Lipids were separated using chloroform/methanol/water (90:10:1, v/v/v) followed by spraying with orcinol and charring. +: presence of GPL-like components, confirmed by their detection after saponification (Fig. S8). −: absence of GPL-like components. C, Deoxyhexose composition analysis of polar lipid fractions by GC/FID and GC/MS. Relative area of identified monosaccharides (n = 4) show Rha residues in green and 6-d-Tal in blue. Identification of 6-d-Tal and Rha was confirmed by the retention time and fragmentation pattern of standards while O-methylated Rha was determined previously (33, 35). Identification of 2- and 3-O-methyl 6-d-Tal was deduced from the retention time and fragmentation pattern. Relative areas of Rha and 6-d-Tal and their methylated forms are expressed as ratios of total monosaccharides.