FIGURE

Figure 2

ID

ZDB-FIG-241114-33

Publication

Aguilera-Correa et al., 2024 - A dTDP-L-Rhamnose 4-epimerase required for glycopeptidolipid biosynthesis in Mycobacterium abscessus

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Figure 2

Enzymatic activity of MAB_4111c.A, reaction catalyzed by MAB_4111c. B, upper panel: HPLC profile of the standards and products formed during the enzymatic reaction in the presence of MAB_4111c or MAB_4111c_Y181A with dTDP-Rha and NADP⁺. The incubation was performed for 60 min. The product of the reaction is labeled with a red box. Lower panel: HPLC profile of standard dTDP-Glc, standard dTDP-6-d-Tal, and dTDP-Glc, and NADP⁺ incubated with MAB_4111c for 60 min. C, upper panel: LC/MS profile of the enzymatic reaction mixture in MRM mode using the specific precursor ion [M-H]⁻ at m/z 547.1. Lower panel: MS² fragmentation spectra of standard dTDP-6-d-Tal and the enzymatic reaction product (∗). D, ¹H NMR spectra of dTDP-L-Rha and NADP⁺; MAB_4111c with dTDP-L-Rha and NADP⁺ after 120 min; MAB_4111c_Y181A with dTDP-L-Rha and NADP⁺ after 120 min; dTDP-6-d-Tal. The characteristic peaks of dTDP-6-d-Tal are indicated with red arrows. E, kinetic analysis of the reaction catalyzed by MAB_4111c in the presence of dTDP-L-Rha and NADP⁺.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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