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Fig. 2

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ZDB-FIG-241113-6

Publication

Jin et al., 2024 - A high-cholesterol zebrafish diet promotes hypercholesterolemia and fasting-associated liver steatosis

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Fig. 2

Effect of HCD on ApoB levels in zebrafish larvae. A: The LipoGlo reporter line, which contains an engineered apoBb.1 gene fused with a nanoluciferase reporter was used for quantifying ApoB-LP levels in fish (21). B: LipoGlo-imaging was used to monitor ApoB-nluc chemiluminescence in whole zebrafish larvae (14 dpf) fed either the control diet or 4% HCD. Twenty-three fish were sampled from each dietary group and a representative image is shown. Scale = 1 mm. C: LipoGlo-counting quantified whole-body ApoB-LP levels in each diet from initial feeding at 6–14 dpf. Each bar represents mean ± SD from 24 fish (robust two-way ANOVA with Games-Howell tests). ∗P < 0.05, ∗∗P < 0.001 D: Comparison of ApoB-LP levels (LipoGlo-counting) in fish fed the HCD and control diet at postprandial or 24 h-fasted stage (n = 16 fish each group, robust two-way ANOVA with Games-Howell tests). ApoB, apolipoprotein B; ApoB-LP, ApoB-containing lipoprotein; dpf, days post fertilization; HCD, high-cholesterol diet.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.