mGluR6a paralog expression in photoreceptor subtype synapses. (A–D′) Z-stacks of adult retinal slices taken with a confocal microscope. The different transgenic lines express GFP under different cone opsin promotors. Anti-PKCα antibody staining labels ON-bipolar cells in blue; in panels A–E′, pink labels mGluR6a puncta; and in panels F–J′, pink labels mGluR6b puncta. (A, A′) LWS1-2 transgenic fish are expressing GFP in their red cones. (B, B′) RH2-2 transgenic fish expressing GFP in green cones. (C, C′) SWS2 transgenic fish expressing GFP in blue cones (*mGluR6a negative; **mGluR6a positive). (D, D′) SWS1 transgenic fish expressing GFP in their UV cones. (E, E′) RH1-3 transgenic fish line expressing GFP in rod photoreceptors. (F, F′) LWS1-2 transgenic fish expressing GFP in their red cones (*mGluR6b negative; **mGluR6b positive). (G, G′) RH2-2 transgenic fish expressing GFP in green cones. (H, H′) SWS2 transgenic fish expressing GFP in their blue cones. (I, I′) SWS1 transgenic fish expressing GFP in their UV cones. (J, J′) RH1-3 transgenic fish line expressing GFP in their rod photoreceptors. The closeups in every image show representative cone-to-ON-bipolar cell synapses for the corresponding staining. Scale bar: 10 µm; scale bar of the closeup: 2 µm.
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