Fig. 2
- ID
- ZDB-FIG-241104-31
- Publication
- Song et al., 2024 - Thymosin β4 promotes zebrafish Mauthner axon regeneration by facilitating actin polymerization through binding to G-actin
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Knockout of tmsb4x attenuates axon regeneration of Mauthner cells. a Experimental workflow diagram. b A representative image of an injured Mauthner axon. The arrow indicates the injury site, which above the cloacal pore. Scale bar, 50 ?m. c Representative images of Mauthner cell axon regeneration in the tmsb4x+/+, tmsb4x+/- and tmsb4?/? lines at 48 hpi. Asterisks indicate the sites of axon injury, and arrows indicate the regenerated axon terminals. Scale bar, 100 ?m d and e Statistical graphs of the length (d) and branch number (e) of regenerated axons in the tmsb4x+/+, tmsb4x+/- and tmsb4?/? lines (lengths: tmsb4x+/+, 402.5 ± 39.5 ?m, n = 14; tmsb4x+/-, 278.6 ± 26.9 ?m, n = 26; tmsb4x?/?, 246.1 ± 26.8 ?m, n = 18; branch numbers: tmsb4x+/+, 1.8 ± 0.2, n = 14; tmsb4x+/-, 1.8 ± 0.2, n = 26; tmsb4x?/?, 1.7 ± 0.2, n = 18). Assessed by one-way ANOVA/Tukey's multiple comparisons test for regeneration length (tmsb4x+/+ vs. tmsb4x+/-, adjusted p = 0.018; tmsb4x+/+ vs. tmsb4x?/?, adjusted p = 0.0047; tmsb4x+/- vs. tmsb4x?/?, adjusted p = 0.71). Kruskal?Wallis test/Dunn's multiple comparisons test was used to assess the branch number (tmsb4x+/+ vs. tmsb4x+/-, adjusted p > 0.99; tmsb4x+/+ vs. tmsb4x?/?, adjusted p > 0.99; tmsb4x+/- vs. tmsb4x?/?, adjusted p > 0.99). The axon regeneration length refers to the distance from the injury site to the tip of the longest regenerating axon branch, as indicated by the distance between the asterisk and the arrow in the figure. Same as below. f Percentages of branch numbers of regenerated axons in the tmsb4x+/+, tmsb4x+/- and tmsb4?/? lines. Sample numbers: tmsb4x+/+, 14; tmsb4x+/-, 26; tmsb4x.?/?,18. g Representative images of axon regeneration with tmsb4x restored in tmsb4x knockout lines. Asterisks indicate the sites of axon injury, and arrows indicate the regenerated axon terminals. Scale bar, 100 ?m. h and i Statistical graphs of the length (h) and branch number (i) of regenerated axons with vector and tmsb4x restored in tmsb4x knockout lines (length: vector, 209.0 ± 33.2 ?m, n = 7; tmsb4x: 422.7 ± 53.1 ?m, n = 9; branch number: vector, 1.9 ± 0.5; n = 7; tmsb4x: 4.4 ± 0.7, n = 9). Assessed by a two-tailed, Mann Whitney test for regeneration length (p = 0.00030). And an unpaired, two-trailed t test was used to assess the branch number. (p = 0.011) |