Fig. 6

Degeneration-regeneration of photoreceptors and changes in visual function following treatment with XAL-NPMOF. (A) Zpr1 and Zpr3 staining in sections taken from retinas of zebrafish in the PBS, NPMOF, XAL and XAL-NPMOF groups at 3 dpl. (B) Quantification of the number of cones and rods in the lesioned retina at 3 dpl. More photoreceptors remained in the XAL-NPMOF group than in the PBS, NPMOF and XAL groups (ANOVA, *p < 0.05). (C) Zpr1 and Zpr3 staining in sections taken from retinas of zebrafish in four groups at 7 dpl. (D) Quantification of the number of cones and rods at 7 dpl. More photoreceptors remained in the XAL-NPMOF group than in the PBS, NPMOF and XAL groups (ANOVA, *p < 0.05). (E) Statistical analysis of the positive correlations of distance and time at 7 dpl. Note that there was a significant decrease in the PBS, NPMOF and XAL groups compared to the normal group in terms of the positive correlation between distance and time (ANOVA, *p < 0.05), while there was no obvious difference between the normal and XAL-NPMOF groups (ANOVA, p > 0.05). Scale bar in (A) and (C): 50 ?m. Abbreviations: dpl, days post lesion; RPE, retinal pigment epithelium; OSL, outer segment layer; ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.