Figure 4

The synergistic effect of promoting motor neurons by the addition of Pgk1 and transfection of various mutations of Eno2. Statistical analysis of the average length of neurites. NSC34 neural cells transfected with siRNA (pCS2+) to inhibit endogenous mouse Eno2 without the addition of extracellular Pgk1 (ePgk1) served as a negative control. As the positive control, NSC34 cells were transfected with eno2 siRNA, followed by transfection of wobble-modified eno2 DNA (eno2-wb) combined with Pgk1 immersion. The resultant average neurite length was normalized as 1.0. Similarly, plasmid harboring a point-mutated eno2 DNA, as indicated, was transfected in siRNA-treated cells, followed by Pgk1 immersion. The average neurite length of motor neurons was measured, and fold change compared to the positive control was calculated. Each experimental group was independently compared with the control group using Student’s t-test for statistical significance (* p < 0.05; ** p < 0.01; ns indicates no significant difference). The t values and degrees of freedom for groups of pCS2⁺, -[M411L], -[D419S] and –[E420K] were 4.972 and 5, 1.151 and 2, 9.281 and 2, and 6.189 and 2, respectively.