Fig. 8
- ID
- ZDB-FIG-241016-36
- Publication
- Wang et al., 2024 - Noncaloric monosaccharides induce excessive sprouting angiogenesis in zebrafish via foxo1a-marcksl1a signal
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Noncaloric monosaccharides induced excessive angiogenesis through foxo1a-marcksl1a signal in zebrafish embryos. (a) A diagram showing the Foxo1 inhibitor and heat shock treatment time window. (b) Real-time PCR analysis of marcksl1a expression in control and AS1842856-treated embryos (n=3). t-test, ***p<0.001. (c) Real-time PCR analysis of marcksl1a expression in control and foxo1a overexpressed embryos (n=3). t-test, **p<0.01. (d) A sequence logo of Foxo1-binding sequence presented in the JASPAR database (https://jaspar.genereg.net/) and two candidate binding sites at the upstream of transcription start site (TSS) of marcksl1a in zebrafish. (e) Results of the chromatin immunoprecipitation (ChIP)-PCR assay indicated that BS1 and BS2 are Foxo1a-binding sites of marcksl1a in zebrafish. Input sonicated genomic DNA samples without immunoprecipitation as a positive control. IgG, sonicated, and IgG-immunoprecipitated genomic DNA samples as a negative control. (f, g) Luciferase reporter activity in foxo1a overexpressed or knocked down embryos (n=3), respectively. t-test, **p<0.01, ****p<0.0001. (h?m) Confocal imaging analysis of blood vessels in control, high glucose, high glucose and Lenvatinib, high glucose+marcksl1a MO, high L-glucose, and high L-glucose+marcksl1a MO groups. (n) Statistical analysis of the total length of intersegmental vessels (ISVs) in the groups in h?m (n=6), respectively. One-way ANOVA, ****p<0.0001. |