FIGURE

Fig. 2

ID

ZDB-FIG-241004-34

Publication

Yen et al., 2024 - Shikonin, a natural naphthoquinone phytochemical, exerts anti-leukemia effects in human CBF-AML cell lines and zebrafish xenograft models

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Effects of SHK on cell cycle distribution, cell apoptosis, and differentiation in Kasumi-1 cells. Cells were treated with vehicle or SHK (0.2 and 0.4 ?M) for 24 and 48 h, and cell cycle progression was detected by flow cytometric analysis. (A) A representative histogram of cell cycle progression. (B) The cell populations in G0/G1, S, and G2/M phases were quantified. (C) Cell apoptosis was detected by flow cytometric analysis. A representative histogram is shown. (D) The population of apoptotic cells was quantified. (E) and (G) Western blot analysis of caspase 3, cleaved caspase 3, Bax, Bcl2, and ?-actin proteins in vehicle- or SHK (0.2 and 0.4 ?M)- treated cells. A representative blot is shown. (F) Normalized intensity of cleaved caspase 3 versus ?-actin proteins. (H) The ratio of Bax versus Bcl-2 proteins. (I) CD11b mRNA expression was measured by RT-qPCR analysis in Kasumi-1 cells treated with vehicle or SHK for 48 h. Data represent the mean � SD of three independent experiments. *p < 0.05 and **p < 0.01 represent significant differences compared to the vehicle-treated cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomed. Pharmacother.