Fig. 1

Effects of SHK on the viability of CBF-AML Kasumi-1 cells. (A) The chemical structure of SHK. (B) Kasumi-1, HL-60, THP-1, Molm-13, U-937 and HEL cells were treated with either vehicle (0.1 % DMSO) or SHK (0.1, 0.2, 0.4, and 0.8 ?M) for 48 h. The cell viability was analyzed using the MTT assay. (C) PBMC were treated with vehicle or SHK (0.1?1.0 ?M) for 48 h. The cell viability was analyzed using the MTT assay. Two AML patient-derived primary PBMCs including (D) AML-M2 #1 PBMC and (E) AML-M2 #2 PBMC were treated with vehicle (0.1 % DMSO) or SHK (0.2 and 0.4 ?M) for 48 h. The cell viability was measured by counting cells after trypan blue staining. The CBF-AML cell lines including (F) Kasumi-1 and (G) SKNO-1 cells were treated with vehicle (0.1 % DMSO) or SHK (0.1?1.0 ?M) for 24 and 48 h. The cell viability was analyzed using the MTT assay. The data represent the mean ± SD of three independent experiments. The *p < 0.05 and **p < 0.01 indicate significant differences compared to the vehicle-treated cells.