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Fig. 2 Effects of SHK on cell cycle distribution, cell apoptosis, and differentiation in Kasumi-1 cells. Cells were treated with vehicle or SHK (0.2 and 0.4 ?M) for 24 and 48 h, and cell cycle progression was detected by flow cytometric analysis. (A) A representative histogram of cell cycle progression. (B) The cell populations in G0/G1, S, and G2/M phases were quantified. (C) Cell apoptosis was detected by flow cytometric analysis. A representative histogram is shown. (D) The population of apoptotic cells was quantified. (E) and (G) Western blot analysis of caspase 3, cleaved caspase 3, Bax, Bcl2, and ?-actin proteins in vehicle- or SHK (0.2 and 0.4 ?M)- treated cells. A representative blot is shown. (F) Normalized intensity of cleaved caspase 3 versus ?-actin proteins. (H) The ratio of Bax versus Bcl-2 proteins. (I) CD11b mRNA expression was measured by RT-qPCR analysis in Kasumi-1 cells treated with vehicle or SHK for 48 h. Data represent the mean ± SD of three independent experiments. *p < 0.05 and **p < 0.01 represent significant differences compared to the vehicle-treated cells.