Fig. 1

The clearance of Tau protein in a zebrafish TAU-overexpressing transgenic model coincides with the increased autophagy activity. a The construct used for the Tau transgenic line that overexpress human TAUP301L and mCherry connected by a self-cleaving 2A peptide under the control of the zebrafish elavl3 promoter and a representative image of zebrafish larvae at 2 dpf. Scale bars = 500 ?m. b Semi-quantitative RT-PCR run on agarose gel shows that human TAU mRNA was steadily expressed at 1, 3, 5, 7 dpf in the Tau tg line. c Western blots with antibodies against total Tau (T46), pTau (AT180, PHF, AT8) and autophagy markers (LC3, p62) for the Ctrl and Tau tg lines at 28 hpf, 2 and 5 dpf. d, e Densitometry measurement of triple biologic replicates of western blots using T46 (d) or p62 (e) antibodies in (c). f Gene expression changes of pro-inflammatory cytokines il6 and tnf-? at 1, 2, and 4 dpf. Data represent mean ± SEM, n = 3 (three biologic replicates), ANOVA test, *p < 0.05; **p < 0.01; ***p < 0.005; ns, not significant. a.u. arbitrary unit