FIGURE

Figure 4

ID

ZDB-FIG-240930-89

Publication

Zhang et al., 2024 - Characterizing Marine Medaka (Oryzias melastigma) Haploid Embryonic Stem Cells: A Valuable Tool for Marine Fish Genetic Research

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Figure 4

Interspecific chimera formation of hMMES1 in a zebrafish host. (A) Micrograph of zebrafish embryo host (n = 200) showing transplanted hMMES1 (red) and MMES1 (green) at 0, 1, and 3 dpf. Scale bars, 100 μm. (B) Molecular analyses of zebrafish transplanted with MMES1 and hMMES1, wild zebrafish, and medaka embryos at day 1–10 (D1–D10) post-fertilization. The ectoderm markers (nf200 and gfap), mesoderm markers (ntl and myf5), endoderm markers (hnf3b and sox17), and neural crest markers (mitf) were amplified by using primers specific to medaka cDNAs; β-actin of marine medaka or zebrafish was amplified as a reference. All experiments were performed three times, and representative data are shown. The uncropped nucleic acid gels figures are presented in the Supplementary Materials.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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